B2261
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B2261
Sigma-Aldrich
bisBenzimide H 33342 trihydrochloride
≥98% (HPLC and TLC)
| Synonym: | bisBenzimide, HOE 33342, Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride |
| CAS Number: | 23491-52-3 |
| Linear Formula: | C27H28N6O · 3HCl · xH2O |
| Molecular Weight: | 561.93 (anhydrous basis) |
| Beilstein Registry Number: | 1234011 |
| EC Number: | 245-691-1 |
| MDL number: | MFCD00012678 |
| PubChem Substance ID: | 24891639 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Excitation max. = 346 nm Emission max. = 460 nm |
| Biochem/physiol Actions | Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA. |
Properties
| assay | ≥98% (HPLC and TLC) |
| solubility | H2O: 20 mg/mL |
| phosphate buffers: precipitates | |
| suitability | passes application test for fluorescence |
| storage temp. | −20°C |
Safety
References
| reference | Crissman, H.A., and Steinkamp, J.A., Exp. Cell Res. 173, 256, (1987) Abstract |
| Latt, S.A. and Stetten, G. J. Histochem. Cytochem. 24, 24, (1976) |
